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Image Search Results
Journal: Cell Reports
Article Title: Importin α5 Regulates Anxiety through MeCP2 and Sphingosine Kinase 1
doi: 10.1016/j.celrep.2018.11.066
Figure Lengend Snippet:
Article Snippet: Briefly, two different short hairpin RNA (shRNA) target sequences from the open reading frame of the mouse importin α5 gene (Kpna1) were cloned into shRNA expression cassettes using BamHI/PacI restriction sites driven by the u6 promoter in a modified third-generation lentiviral plasmid with hUBC-driven
Techniques: Plasmid Preparation, Recombinant, RNA Extraction, Purification, Expressing, Next-Generation Sequencing, shRNA, Software, Binding Assay